Team:Warsaw/Calendar-Main/21 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
<h3>Michał L., Ewa, Marcin</h3>
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<h4> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr"><a>PCR</a> </h4>
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<h4><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a></h4>
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Revision as of 18:51, 1 October 2008

Gallery Bricks Notebook Team Project Home


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Michał L., Ewa, Marcin

PCR

ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 + AP+NotI 470 bp
alpha-linkerpUC19AlphaL+SacI + AlphaP+link10+homo2 1022 bp
omega-linkerpUC19OmegaL+SacI + OmegaP+link10+homo2 779 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: homemade Pfu-Turbo

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