Team:Warsaw/Calendar-Main/21 October 2008

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Revision as of 23:53, 26 October 2008


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Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA_omega_ (from 16 October) to LB with kanamycin.

Inoculation of colonies from plate with ligation of pACYC177 + OmpA_alpha_ to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.
Isolation of plasmids from cultures inoculated on previous day RFP(?????) + AraC+pBAD+AID and 5kb(?????) + pT7_alpha.
Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found for 5kb(?????) + pT7_alpha.

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+<h3>Preparation of pACYC177 + OmpA_omega_</h3>
+<h4>Piotr</h4>
+<p> Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA_omega_ (from 16 October) to LB with kanamycin. </p>
 <h3>Preparation of BioBricks</h3>
 <h4>Michał K.</h4>
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-<h3></h3>
-<h4>Piotr</h4>
-<p> Inoculation of few more colonies from plate with ligation of pACYC177 + OmpA_omega_ (from 16 October) to LB with kanamycin. </p>
 </html>
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