Team:Warsaw/Calendar-Main/22 May 2008

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1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>
1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>

Revision as of 22:00, 30 September 2008

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Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.:
1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

20 cycles