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Team:Warsaw/Calendar-Main/22 May 2008
From 2008.igem.org
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Michał K.:<br> | Michał K.:<br> | ||
| - | 1. PCR - transcription fusion: AID + T7 RNA-polymerase | + | 1. PCR - transcription fusion: AID + T7 RNA-polymerase |
<p>Primers: </p> | <p>Primers: </p> | ||
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20 cycles <br> | 20 cycles <br> | ||
| - | 2. PCR - translation fusion: AID + T7 RNA-polymerase | + | 2. PCR - translation fusion: AID + T7 RNA-polymerase |
<p>Primers: </p> | <p>Primers: </p> | ||
Revision as of 22:16, 30 September 2008
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Piotr: 1. Design of primers for sequencing something we want from pMPM-T5 Primers: Michał K.: Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature : 60°C 20 cycles 2. PCR - translation fusion: AID + T7 RNA-polymerase Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature : 60°C 20 cycles
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