Team:Warsaw/Calendar-Main/22 May 2008

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Revision as of 13:03, 1 October 2008

Gallery Bricks Notebook Team Project Home


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Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.:
1. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

20 cycles
2. Gel electrophoresis and isolation of proper bands (3300 bp).