Team:Warsaw/Calendar-Main/22 May 2008

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<h4>Piotr:</h4>
<h4>Piotr:</h4>
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Design of primers for sequencing something we want from pMPM-T5<br>
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Design of primers for sequencing something we want from pMPM-T5</li><br>
Primers:<br>
Primers:<br>
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Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br>
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br>
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Elongation temperature : 73&deg;C <br>
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Annealing temperature : 73&deg;C <br>
Elongation time: 4 minutes <br>
Elongation time: 4 minutes <br>

Revision as of 21:36, 1 October 2008

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Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5

    2. Primers:

    Michał K.:

    1. PCR - translation fusion: AID + T7 RNA-polymerase
      Primers:
      Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
      Annealing temperature : 73°C
      Elongation time: 4 minutes
      20 cycles
    2. Gel electrophoresis and isolation of proper band (3300 bp).

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