Team:Warsaw/Calendar-Main/22 May 2008

From 2008.igem.org

(Difference between revisions)
Line 29: Line 29:
-
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion <br>
+
Template DNA: purified PCR products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> - AID and T7 RNA-polymerase for translation fusion <br>
Annealing temperature : 73&deg;C <br>
Annealing temperature : 73&deg;C <br>

Revision as of 19:40, 4 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5.

    2. Primers:

    Michał K.:

    1. PCR - translation fusion: AID + T7 RNA-polymerase
      Primers:
      Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
      Annealing temperature : 73°C
      Elongation time: 4 minutes
      20 cycles
    2. Gel electrophoresis and isolation of proper band (3300 bp).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008"