Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Piotr:

1. Design of primers for sequencing something we want from pMPM-T5.



Primers:


• pMPMT5seqF
• pMPMT5seqR
• AIDseqP

Michał K.:

1. Isolation of hypothetical pMPM-T5 with transcription fusion.
2. Isolation of hypothetical pMPM-T5 with translation fusion.
3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).
4. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).
5. Gel electrophoresis.