Team:Warsaw/Calendar-Main/22 May 2008

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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).</li>
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI(BamHI buffer).</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li>
<li> Gel electrophoresis (no proper colonies found).</li></html>
<li> Gel electrophoresis (no proper colonies found).</li></html>
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Revision as of 15:06, 5 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5.

  2. Primers:

Michał K.:

  1. Isolation of hypothetical pMPM-T5 with transcription fusion.
  2. Isolation of hypothetical pMPM-T5 with translation fusion.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translation fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (no proper colonies found).