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Team:Warsaw/Calendar-Main/22 May 2008
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<li> Gel electrophoresis (no proper colonies found).</li> | <li> Gel electrophoresis (no proper colonies found).</li> | ||
<img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/> | <img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/> | ||
| - | <h3>1-DNA ladder; 2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); 4-pMPM-T5-AID digested with EcoRI; 5-hypothetical pMPM with translation fusion digested with BamHI; 6-pMPM-T5-AID digested with BamHI.</h3> | + | <h3>1-DNA ladder;<br> |
| + | 2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); <br> | ||
| + | 4-pMPM-T5-AID digested with EcoRI; <br> | ||
| + | 5-hypothetical pMPM with translation fusion digested with BamHI; <br> | ||
| + | 6-pMPM-T5-AID digested with BamHI.</h3> | ||
</html> | </html> | ||
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Revision as of 08:09, 6 October 2008
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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Piotr:
Primers: Michał K.:
1-DNA ladder;
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