Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.:
1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

20 cycles

2. PCR - translation fusion: AID + T7 RNA-polymerase</p>

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature : 60°C
Annealing time: 4 minutes

20 cycles
3. Gel electrophoresis and isolation of proper bands