Piotr:

Design of primers for sequencing something we want from pMPM-T5
Primers:

• pMPMT5seqF
• pMPMT5seqR
• AIDseqP

Michał K.:

1. PCR - translation fusion: AID + T7 RNA-polymerase
   Primers:
   
   • AIDlNrH
   • T7pXbSal
   Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
   Elongation temperature : 73°C
   Elongation time: 4 minutes
   20 cycles
   
2. Gel electrophoresis and isolation of proper band (3300 bp).