Team:Warsaw/Calendar-Main/22 May 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5.

    2. Primers:

Michał K.:

  1. Isolation of hypothetical pMPM-T5 with transcription fusion.
  2. Isolation of hypothetical pMPM-T5 with translation fusion.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translation fusion with BamHI(BamHI buffer).
  5. Gel electrophoresis (no proper colonies found).

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