Team:Warsaw/Calendar-Main/22 October 2008

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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_I739204>pACYC177</a> + <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>

Revision as of 10:42, 27 October 2008

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Preparation of BioBricks

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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