From 2008.igem.org
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| - | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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| - | Paweł</h3>
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| - | <p><ol>
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| - | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inocluated on previous day</li>
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| - | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~150 bp visible)</li>
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| - | <li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of pET15b+Z+omega vector</li>
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| - | </ol></p>
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| | <h3>Antoni</h3> | | <h3>Antoni</h3> |
Revision as of 19:36, 11 October 2008
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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
- Results of ligation: 6 colonies grown.
- Each colony cultured overnight in LB + ampicillin.
Antoni
- Setup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria.
Cloning of protein A DNA to OmpA constructsMichał K.
- Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
- Control digest of isolated plasmids with BamHI and SacI.
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