Team:Warsaw/Calendar-Main/23 July 2008

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
<br>
<br>
</ol>
</ol>
-
 
-
 
-
 
-
 
-
<h3>Antoni</h3>
 
-
<p>
 
-
<ol><li>Setup of overnight culture <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> (LB broth) for preparation of chemocompetent bacteria.</li></ol>
 
-
</p>
 
-
 
-
 
<h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4>
<h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4>
Line 26: Line 16:
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI. </li></ol>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI. </li></ol>
 +
<h3>Antoni</h3>
 +
<p>
 +
Setup of overnight culture <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> (LB broth) for preparation of chemocompetent bacteria.</p>
-
<html>
 
</html>
</html>

Revision as of 19:38, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.

Cloning of protein A DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
  2. Control digest of isolated plasmids with BamHI and SacI.

Antoni

Setup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/23_July_2008"