Team:Warsaw/Calendar-Main/23 June 2008

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The analysis of beta-galactosidase sequence from pZC

Piotr

There is no mutation in beta-galactosidase gene. Why the colonys are white??? This is wery strange... We must make new reporter system.

Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpha-A and omega-A fusions

Michał L.

We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg²⁺ concentration elevated to 4 mM.

Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.