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Team:Warsaw/Calendar-Main/23 May 2008
From 2008.igem.org
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1. Digest of pMPM-T5 with NcoI and XbaI 2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI 3. Clean-up of digested products from p.1 and p.2 4. Ligation of digested products from p.1 and p.2 5. Transformation of E.coli TOP10 with ligation product 6. Digest of pMPM-T5+AID with HindIII and SalI 7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI 8. Clean-up of digested products from p.6 and p.7 9. Ligation of digested products from p.6 and p.7 10. Transformation of E.coli TOP10 with ligation product 11. Transformants plating on LB + tetracycline
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