Team:Warsaw/Calendar-Main/24 July 2008

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1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.

Antoni:

1. Preparation of chemocompetent bacteria E. coli TOP10.

Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

  1. Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha dephosphorylation with CIAP
  2. Gel electrophoresis of digested plasmids and gel-out of proper bands
  3. Ligation of pET15b+alfa and A

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