Team:Warsaw/Calendar-Main/24 June 2008
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<h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b-OmpA-alpha</a> in place of OmpA</h3> | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b-OmpA-alpha</a> in place of OmpA</h3> | ||
- | <h4>Piotr, | + | <h4>Piotr, Antoni</h4> |
<p><ol> | <p><ol> | ||
<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> |
Revision as of 20:31, 19 October 2008
Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinStill no success. We need to run gradient PCR to find optimal reaction conditions. Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, Antoni
Preparation of constructs with OmpA protein fusionsMichał K.
Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.
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