Team:Warsaw/Calendar-Main/25 July 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
 
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>
 
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin
 
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Revision as of 23:27, 2 October 2008

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1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).


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