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Team:Warsaw/Calendar-Main/26 June 2008
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<tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
<tr><td>48°C-62°C</td><td>0:45</td></tr> | <tr><td>48°C-62°C</td><td>0:45</td></tr> | ||
| - | <tr><td> | + | <tr><td>68°C</td><td>2:30</td></tr> |
| - | <tr><td> | + | <tr><td>68°C</td><td>10:00</td></tr> |
<tr><td>4°C</td><td>infinite</td></tr> | <tr><td>4°C</td><td>infinite</td></tr> | ||
</table> | </table> | ||
Revision as of 12:36, 10 October 2008
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Michał K. Michał L., Ewa, Marcin |
| Product | Templates | Primers | Product length |
|---|---|---|---|
| Alpha-A fusion | Alpha-linker + linker-A | AlphaL+SacI + AP+NotI | 1500 bp |
| Omega-A fusion | Omega-linker + linker-A | OmegaL+SacI + AP+NotI | 1440 bp |
PCL program for fusion A with alpha and omega
| Temperature | Time | |
|---|---|---|
| 94°C | 4:00 | |
| 94°C | 0:30 | 28 cycles |
| 48°C-62°C | 0:45 | |
| 68°C | 2:30 | |
| 68°C | 10:00 | |
| 4°C | infinite |
