Team:Warsaw/Calendar-Main/26 June 2008

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<table id="result">
<table id="result">
<tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr>
<tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr>
-
<tr><th>Alpha-A fusion</th><td>Alpha-linker + linker-A</td><td>AlphaL+SacI + AP+NotI </td><td>1500 bp</td></tr>
+
<tr><th>Alpha-A fusion</th><td>Alpha-linker + linker-A</td><td>AlphaL+SacI + AP+NotI </td><td>1100 bp</td></tr>
-
<tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr>
+
<tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>750 bp</td></tr>
</table>
</table>
<br><br>
<br><br>

Revision as of 18:45, 19 October 2008

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Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

  1. Isolation of pZC
  2. Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)

Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs, gel-out isolations and digests from 17 June.

Polymerase Chain Ligation (PCL)

Michał L., Ewa, Marcin

ProductTemplatesPrimersProduct length
Alpha-A fusionAlpha-linker + linker-AAlphaL+SacI + AP+NotI 1100 bp
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 750 bp


PCL program for fusion A with alpha and omega
TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-62°C0:45
68°C2:30
68°C10:00
4°Cinfinite

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