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Team:Warsaw/Calendar-Main/26 September 2008
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Mutagenesis of protein A
Paweł
Results of mutageneses:
ADelL+KpnI and ADelP primers mutagenesis:
pACYClac+OmpA-ΔA-omega
:
5
colonies
pACYClac+OmpA-ΔA-alpha
:
10
colonies
pACYClac+OmpA-omega-ΔA
:
3
colonies
AMutL+KpnI and AMutP mutagenesis:
pACYClac+OmpA-ΔA-omega
:
20
colonies
pACYClac+Ompa-ΔA-alpha
:
12
colonies
pACYClac+OmpA-omega-ΔA
:
17
colonies
Each colony cultured overnight in LB + kanamycin for further analyses.
Preparation of
ΔA (BBa_K103003)
Piotr
Isolation
of plasmid from culture inoculated on previous day.
Control
digest
of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.