Team:Warsaw/Calendar-Main/27 September 2008

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Mutagenesis of protein A

Paweł

  1. Isolation of plasmid DNA from overnight cultures (it was really tough work...).
  2. Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
  3. Results of digest:
  4. Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).