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Team:Warsaw/Calendar-Main/28 July 2008
From 2008.igem.org
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<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p> | <p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p> | ||
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| + | <h3>Protein purification: Z-alpha i Z-omega</h3> | ||
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| + | <h4>Piotr</h4> | ||
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| + | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.</p></html> | ||
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Revision as of 20:53, 19 October 2008
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, MarcinSequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. Protein purification: Z-alpha i Z-omegaPiotrSingle transformations of Rosetta with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.
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