Team:Warsaw/Calendar-Main/29 August 2008

From 2008.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 9: Line 9:
<h4>Piotr</h4>
<h4>Piotr</h4>
<p>
<p>
-
Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a>. Plating on LB with chloramphenicol and ampicillin.</p>
+
Transformation of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a> plasmid. Plating on LB with chloramphenicol and ampicillin.</p>
<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3>
<h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3>
Line 31: Line 31:
https://static.igem.org/mediawiki/2008/a/a8/Kokodak.jpg" width=300/></a> <var><b>Fig. 1.</b> Control XbaI/BamHI digests of  plasmids isolated from transformants with colony PCR product.<br>  
https://static.igem.org/mediawiki/2008/a/a8/Kokodak.jpg" width=300/></a> <var><b>Fig. 1.</b> Control XbaI/BamHI digests of  plasmids isolated from transformants with colony PCR product.<br>  
1. Marker<br>
1. Marker<br>
-
2-4. digested plasmids pET15b+A-alpha <br></var>
+
2-4. Digested plasmids pET15b+A-alpha <br></var>

Latest revision as of 20:58, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha

Piotr

Transformation of Rosetta with pET15b+A-alpha plasmid. Plating on LB with chloramphenicol and ampicillin.

Blue-white screening and rifampicin test in GM2163

Michał L.

GM2163 competent cells carrying pZC320 were transformed with following plasmids:

Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Isolation of pET15b+A-alpha plasmids.
  2. Control digest of isolated plasmids with XbaI and BamHI (Tango buffer).
  3. Gel electrophoresis (Fig. 1.). Choice of proper clone.

Fig. 1. Control XbaI/BamHI digests of plasmids isolated from transformants with colony PCR product.
1. Marker
2-4. Digested plasmids pET15b+A-alpha



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008"