Team:Warsaw/Calendar-Main/29 August 2008

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Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Isolation of pET15b+OmpA-alfa.

Protein purification: A-alpha, Z-alpha i Z-omega
Piotr

  1. Single transformations of Rosetta with plasmids pET15b+A-alfa, pET15b+Z-alfa and pET15b+Z-omega. Plating them on LB with Cm and Ap.