Team:Warsaw/Calendar-Main/29 May 2008

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<h3>PCR results of translational fusion for the A+AT construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
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https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg
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Revision as of 20:54, 20 September 2008

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1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB

2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose

3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB

4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG

5. Wait for 5 hours

6. OD measurment

7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + riphampicin)


PCR results of translational fusion for the A+AT construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

Tranlacyjna_pcr_29_05_2008.jpg