Team:Warsaw/Calendar-Main/2 July 2008

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<h3>Change of the reporter from pZC with B-galactosidaze to GFP or RFP</h3>
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<h4>Piotr, Weronika</h4>
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Gel electrophoresis and gel out of proper bands
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Ligation of pZC with nazwa (Gfp genetrator), nazwa (RFP generator)
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<li>Chemotransformation of E.coli TOP10 with ligation products</li>
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<li>Plating transformants on LB+Amp30+X-gal+IPTG
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<h3>Preparation of constructs with OmpA protein fusions</h3>
<h3>Preparation of constructs with OmpA protein fusions</h3>

Revision as of 09:24, 16 October 2008

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Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

  1. Gel electrophoresis and gel out of proper bands
  2. Ligation of pZC with nazwa (Gfp genetrator), nazwa (RFP generator)
  3. Chemotransformation of E.coli TOP10 with ligation products
  4. Plating transformants on LB+Amp30+X-gal+IPTG

    Preparation of constructs with OmpA protein fusions

    Paweł

    1. Clean-up of overnight digest.
    2. Ligation of OmpA_alfa and pET15b.
    3. Ligation of OmpA_omega and pET15b.
    4. Transformation of E. coli TOP10 strain with ligation products.
    5. Transformants plating on LB + ampicillin.

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