Team:Warsaw/Calendar-Main/2 June 2008

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<table id="result">
<table id="result">
<tr><th>Strain</th><th>Inducer</th><th>Number of colonies on rifampicin</th><th>OD</th></tr>
<tr><th>Strain</th><th>Inducer</th><th>Number of colonies on rifampicin</th><th>OD</th></tr>
-
<tr><th>Top10</th><td>none</td><td>9</td><td>2.66</td></tr>
+
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a></th><td>none</td><td>9</td><td>2.66</td></tr>
-
<tr><th>Top10 pMPM-AID</th><td>none</td><td>15</td><td>2.5</td></tr>
+
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>none</td><td>15</td><td>2.5</td></tr>
-
<tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>42</td><td>2.27</td></tr>
+
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a></th><td>0.1% Arabinose</td><td>42</td><td>2.27</td></tr>
-
<tr><th>Top10 pTRC-AID</th><td>none</td><td>12</td><td>2.41</td></tr>
+
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>none</td><td>12</td><td>2.41</td></tr>
-
<tr><th>Top10 pTRC-AID</th><td>0.5 mM IPTG</td><td>35</td><td>1.98</td></tr>
+
<tr><th><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a></th><td>0.5 mM IPTG</td><td>35</td><td>1.98</td></tr>
</table>
</table>
<br>
<br>

Revision as of 09:37, 25 October 2008

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Results of rifampicin test and OD measurement

Piotr

StrainInducerNumber of colonies on rifampicinOD
TOP10none92.66
TOP10 pMPM-T5-AIDnone152.5
TOP10 pMPM-T5-AID0.1% Arabinose422.27
TOP10 pTrc99a-AIDnone122.41
TOP10 pTrc99a-AID0.5 mM IPTG351.98

Conclusion: AID seems to work, induction of AID may reduces the growth rate

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

PCR for AID transcriptional fusion with translational fusion AID+T7 RNA-polymerase.
Primers: AIDlNrH and T7pXbSal
Template DNA: translational fusion AID+T7 RNA-polymerase (pMPMT5+AID-T7)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20 and 25

Blue/white and rifampicin test

Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin (30 μg/mL) + X-gal + IPTG.

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