Piotr:
1.Transformation of E.coli TOP10 with pZC320.

2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p>

Michał K.:

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers:

<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
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Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

Number of cycles: 20