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Team:Warsaw/Calendar-Main/2 October 2008
From 2008.igem.org
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primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/Part:BBa_K103006>OmpA-linker (BBa_K103006)</a> (annealing temperature - 55°C,45 s of elongation step). </li> | primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/Part:BBa_K103006>OmpA-linker (BBa_K103006)</a> (annealing temperature - 55°C,45 s of elongation step). </li> | ||
| - | <li> Gel electrophoresis.</li> | + | <li> Gel electrophoresis(<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008#fig1>Fig. 1.</a>).</li> |
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.</li> | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.</li> | ||
</ol> | </ol> | ||
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<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103004>Z(BBa_K103004)</a>).</li> | <ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from culture inoculated on previous day (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103004>Z(BBa_K103004)</a>).</li> | ||
| - | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li></ol> | + | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmid with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found (<li> Gel electrophoresis(<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008#fig2>Fig. 2.</a>).</li></ol> |
<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/6/6f/Traw_02_10_2008.jpg"></a> | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/6/6f/Traw_02_10_2008.jpg"></a> | ||
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<ol> | <ol> | ||
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=http://partsregistry.org/Part:BBa_K103018>BBa_K103018</a> fragment with EcoRI and BcuI (BamHI buffer). </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=http://partsregistry.org/Part:BBa_K103018>BBa_K103018</a> fragment with EcoRI and BcuI (BamHI buffer). </li> | ||
| - | <li>Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 1200 bp. </li> | + | <li>Gel electrophoresis <li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008#fig3>Fig. 3.</a>) and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 1200 bp. </li> |
</ol> | </ol> | ||
Revision as of 19:26, 28 October 2008
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Preparation of linker_alpha (BBa_K103009)Michał K.Overnight ligation of isolated DNA fragments: pSB2K3 + linker_alpha (BBa_K103009). Preparation of linker_omega (BBa_K103013)Michał K.Overnight ligation of isolated DNA fragments: pSB2K3 + linker_omega (BBa_K103013). Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.Overnight ligation of DNA fragments isolated on 30 September : pACYC177 + OmpA-linker-omega-linker (BBa_K103016). Preparation of OmpA-linker (BBa_K103006)Michał K.
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