Team:Warsaw/Calendar-Main/30 July 2008
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<p>1. Isolation of plasmids from cultures inocluated on previous day.<br> | <p>1. Isolation of plasmids from cultures inocluated on previous day.<br> | ||
2. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). </p> | 2. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). </p> | ||
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+ | Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr | ||
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+ | Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50 | ||
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+ | wieczorem mieżenie OD : tabelka | ||
Revision as of 13:37, 5 October 2008
1. Isolation of plasmids from cultures inocluated on previous day.
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