Team:Warsaw/Calendar-Main/30 July 2008
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- | <h3>Checking if OmpA_omega_A_alpha gives ampicillin resistance < | + | <h3>Checking if OmpA_omega_A_alpha gives ampicillin resistance </h3> |
- | Piotr</ | + | <h4>Piotr</h4> |
<p>Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mM into same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100 mM) in ratio 1:50.</p> | <p>Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mM into same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100 mM) in ratio 1:50.</p> |
Revision as of 22:34, 10 October 2008
Checking if OmpA_omega_A_alpha gives ampicillin resistancePiotrInoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mM into same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100 mM) in ratio 1:50.
Conclusion: we obtained the best induction using 0.5 - 0.25 mM IPTG. Best ampicillin concentration is 50 - 75 μg/ml.
Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases. (present in top10)
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