Team:Warsaw/Calendar-Main/30 July 2008

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<h3>Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha</h3><h4>Michał K.</h4>
<ol><li>Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.  
<ol><li>Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.  
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<h4>Michał K.:</h4>
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<li>Isolation of plasmids from cultures inocluated on previous day.</li>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). </li></ol></p>
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Revision as of 21:51, 11 October 2008

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Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha

Michał K.

  1. Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.


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