Team:Warsaw/Calendar-Main/30 July 2008
From 2008.igem.org
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Primers: | Primers: | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> | ||
- | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> <br> |
Elongation time: 30s <br> | Elongation time: 30s <br> | ||
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<li>Gel electrophoresis and isolation of 250 bp band. </li> | <li>Gel electrophoresis and isolation of 250 bp band. </li> | ||
- | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of isolated PCR product, pACYC177+OmpA_alpha and pACYC177+OmpA_omega with NotI and SacI (BamHI bauffer). pACYC177 vectors were | + | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of isolated PCR product, pACYC177+OmpA_alpha and pACYC177+OmpA_omega with NotI and SacI (BamHI bauffer). pACYC177 vectors were also <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylated</a>. </li> |
<li>Clean-up of digestion reaction. </li> | <li>Clean-up of digestion reaction. </li> | ||
<li>Gel electrophoresis for estimation of DNA concentration. </li> | <li>Gel electrophoresis for estimation of DNA concentration. </li> |
Revision as of 23:58, 11 October 2008
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin. Cloning of truncated fragment of protein A DNAMichał K.
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