Team:Warsaw/Calendar-Main/30 July 2008
From 2008.igem.org
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MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
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<h3> Cloning of truncated fragment of protein A DNA</h3> | <h3> Cloning of truncated fragment of protein A DNA</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
- | <ol><li>Optimization of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain truncated fragment of protein A DNA. | + | <ol><li>Optimization of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain truncated fragment of protein A DNA.<br> |
Primers: | Primers: | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a> |
Revision as of 00:03, 12 October 2008
Cloning of omega_A DNA fragment to pACYC177+OmpA_alphaMichał K.Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin. Cloning of truncated fragment of protein A DNAMichał K.
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