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From 2008.igem.org

Attribution analyzer / Interactive list of topics   1. Isolation of plasmids from cultures inocluated on previous day. 2. Control digest of isolated plasmids (pACYC177+omega_deltaA) with HindIII and SacI (7 from 8 colonies confirmed). Piotr Checking if OmpA_omega_A_alpha gives ampicillin resistance Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 into the same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100) in relation 1:50 Bacterial growth (OD) measurement in the evening: ampicillin concentration (μg/mL): IPTG concentration (mmol/mL): 0 0,1 0,25 0,5 0,75 1 25 1,054 1,154 1,051 0,99 1,096 0,896 50 0,94 0,891 1,123 0,847 0,924 0,81 75 0.5 0,63 0.743 0.782 0.631 0.64 100 0.02 0,396 0.563 0.678 0.602 0.611 Wniosek: najlepsz indukcja jest 0,5 - 0,25 mM IPTG, a ampicyliny dawać należy 50 - 75 mikrograma na mililitr Ampicyliuny Checking if degradation of fusion with OmpA is a result of the activity of lon and iompt proteases. (present in top10) Transformation omega_A_alpha and A_alpha into Rosetta.

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