Team:Warsaw/Calendar-Main/3 September 2008
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<li>A-alpha inoculated for overnight culture (Rosetta strain).</li></ol></p> | <li>A-alpha inoculated for overnight culture (Rosetta strain).</li></ol></p> | ||
- | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/b/bd/September_3_rd.jpg" width=300 /></a><var>Fig. 1.</ | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/b/bd/September_3_rd.jpg" width=300 /></a><var><b>Fig. 1.</b> Supernatants from A-alpha induction with various IPTG concentrations<br> |
The arrow shows place of our overexpressed protein:<br> | The arrow shows place of our overexpressed protein:<br> | ||
1. 22°C 0.5 mM IPTG 5 h<br> | 1. 22°C 0.5 mM IPTG 5 h<br> |
Revision as of 19:36, 26 October 2008
Purification of proteins: A-alphaPiotr, Emilia
The arrow shows place of our overexpressed protein: 1. 22°C 0.5 mM IPTG 5 h 2. Marker 3. 22°C 0.5 mM IPTG overnight 4. 22°C 1 mM IPTG 5 h 5. 22°C 1 mM IPTG overnight 6. 37°C 0.5 mM IPTG 5 h 7. 37°C 0.5 mM IPTG overnight 8. 37°C 1 mM IPTG 5 h 9. 37°C 1 mM IPTG overnight 10. Marker
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