Team:Warsaw/Calendar-Main/4 August 2008

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<h3>Checking if omp_omega_A_alpha gives ampicillin resistance<br>
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<h3>Checking if omp_omega_A_alpha gives ampicillin resistance<h4>Piotr</h4>
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Piotr</h3>
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<ol><li>Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL</li></ol>
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<p>Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.</p>

Revision as of 12:37, 12 October 2008

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Cloning of truncated fragment of protein A

Piotr

Inoculation of some pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA colonies of tranformnts.

Checking the expression of omp_omega_A_alpha and omp_A_alpha

Piotr

Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.

Checking if omp_omega_A_alpha gives ampicillin resistance

Piotr

Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL.