Team:Warsaw/Calendar-Main/5 June 2008

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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a><br>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a><br>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqP">pZCseqP</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqP">pZCseqP</a>
 +
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPMT5 with T7 RNA polymerase.</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPMT5+T7  with BamHI and SalI (BamHI buffer).</li>
</p></ol>
</p></ol>

Revision as of 18:30, 5 October 2008

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Michał L., Ewa, Marcin:

  1. Inoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin).

Piotr:

  1. Design of primers for sequencing β-galactosidase from pZC320.

    2. Primers:
    pZCseqL
    pZCseqP
  2. Isolation of hypothetical pMPMT5 with T7 RNA polymerase.
  3. Control digest of pMPMT5+T7 with BamHI and SalI (BamHI buffer).

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