"
Team:Warsaw/Calendar-Main/5 June 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 6: | Line 6: | ||
<ol> <li> | <ol> <li> | ||
Inoculation of of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> into liquid LB broth (with 30 μg/ml of ampicillin).</li></ol><br> | Inoculation of of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> into liquid LB broth (with 30 μg/ml of ampicillin).</li></ol><br> | ||
| - | <h4>Piotr:</h4><ol> <li> | + | |
| + | <h3>Preparation of pMPMT5+T7 construct</h3> | ||
| + | <h4>Piotr:</h4><ol> | ||
| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPMT5 with T7 RNA polymerase.</li> | ||
| + | |||
| + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPMT5+T7 with BamHI and SalI (BamHI buffer).</li> | ||
| + | <li> | ||
<p>Design of primers for sequencing β-galactosidase from <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.<br></li> | <p>Design of primers for sequencing β-galactosidase from <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.<br></li> | ||
| Line 14: | Line 20: | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a><br> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a><br> | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqP">pZCseqP</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqP">pZCseqP</a> | ||
| - | |||
| - | |||
</p></ol> | </p></ol> | ||
Revision as of 18:32, 5 October 2008
 |
|
||||||||
 |
|
||||||||
|
|
