Team:Warsaw/Calendar-Main/5 June 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPMT5 with T7 RNA polymerase.</li>
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPMT5 with T7 RNA polymerase.</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPMT5+T7 with BamHI and XbaI (1x Tango buffer).</li>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + T7 RNA-polymerase with EcoRI.</li>
<li> Choice of proper clones and preparation for sequencing.</li>
<li> Choice of proper clones and preparation for sequencing.</li>

Revision as of 18:45, 5 October 2008

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Michał L., Ewa, Marcin:

  1. Inoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin).

Preparation of pMPMT5+T7 construct

Piotr:

  1. Isolation of hypothetical pMPMT5 with T7 RNA polymerase.
  2. Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
  3. Choice of proper clones and preparation for sequencing.

  4. Design of primers for sequencing β-galactosidase from pZC320.

    5. Primers:
    pZCseqL
    pZCseqP


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