Team:Warsaw/Calendar-Main/5 June 2008

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Design of primers for sequencing β-galactosidase from <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</p>
Design of primers for sequencing β-galactosidase from <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>.</p>
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Primers:  
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<p>Primers:  

Revision as of 10:16, 11 October 2008

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Preparation of pMPMT5+T7 construct

Piotr

  1. Isolation of hypothetical pMPMT5 with T7 RNA polymerase.
  2. Control digest of pMPMT5 + T7 RNA-polymerase with EcoRI (EcoRI buffer).
  3. Gel electrophoresis.
  4. Choice of proper clone and preparation for sequencing.

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

Inoculation of colonies of transformants (pMPMT5-AID with AID-T7 RNA-polymerase translation fusion) into liquid LB+tetracycline.

Blue/white and rifampicin test

Michał L., Ewa, Marcin

Inoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin).


Piotr

Design of primers for sequencing β-galactosidase from pZC320.

Primers: pZCseqL and pZCseqP

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