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Team:Warsaw/Calendar-Main/5 June 2008
From 2008.igem.org
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| - | <h3>Preparation of pMPMT5+T7 construct</h3> | + | <h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-T7>pMPMT5+T7</a> construct</h3> |
<h4>Piotr</h4><ol> | <h4>Piotr</h4><ol> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPMT5 with T7 RNA polymerase.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-T7>pMPMT5 with T7 RNA polymerase</a>.</li> |
| - | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPMT5 + T7 RNA-polymerase with EcoRI (EcoRI buffer).</li> | + | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-T7>pMPMT5 + T7 RNA-polymerase </a>with EcoRI (EcoRI buffer).</li> |
<li> Gel electrophoresis.</li> | <li> Gel electrophoresis.</li> | ||
Revision as of 16:58, 12 October 2008
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Preparation of pMPMT5+T7 constructPiotr
Preparation of pMPMT5-AID+AIDT7 constructMichał K.Inoculation of colonies of transformants (pMPMT5-AID with AID-T7 RNA-polymerase translation fusion) into liquid LB+tetracycline. Blue/white and rifampicin testMichał L., Ewa, MarcinInoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin). PiotrDesign of primers for sequencing β-galactosidase from pZC320.
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