Michał L., Ewa, Marcin:

1. Inoculation of of E. coli TOP10 with pZC320 into liquid LB broth (with 30 μg/ml of ampicillin).

Preparation of pMPMT5+T7 construct

Piotr:

1. Isolation of hypothetical pMPMT5 with T7 RNA polymerase.
2. Control digest of pMPMT5 + T7 RNA-polymerase with EcoRI.
3. Choice of proper clone and preparation for sequencing.

4. Design of primers for sequencing β-galactosidase from pZC320.
   

Primers:
pZCseqL
pZCseqP