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Team:Warsaw/Calendar-Main/6 July 2008
From 2008.igem.org
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| + | <h3>Cloning omega-A fusion on pKS (second attempt)</h3> | ||
| + | <h4>Michał L., Ewa, Marcin:</h4> | ||
| + | <ol> | ||
| + | <li>Gel electrophoresis of PCR products</li> | ||
| + | <li>Gel-out of proper bands</li> | ||
| + | <li>PCL reaction to create omega-A fusion:</li> | ||
| + | </ol> | ||
| + | |||
| + | <table id="result"> | ||
| + | <tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr> | ||
| + | <tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>1440 bp</td></tr> | ||
| + | </table> | ||
| + | |||
| + | <h4>PCL program for omega-A fusion</h4> | ||
| + | |||
| + | <table id="result"> | ||
| + | <tr><th>Temperature</th><th>Time</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>48°C-58°C</td><td>0:45</td></tr> | ||
| + | <tr><td>68°C</td><td>2:00</td></tr> | ||
| + | <tr><td>68°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
| + | </html> | ||
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Revision as of 13:58, 10 October 2008
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin:
PCL program for omega-A fusion
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