Team:Warsaw/Calendar-Main/6 July 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

  1. Gel electrophoresis of PCR products
  2. Gel-out of proper bands
  3. PCL reaction to create omega-A fusion:

ProductTemplatesPrimersProduct length
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp

PCL program for omega-A fusion

TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-58°C0:45
68°C2:00
68°C10:00
4°Cinfinite

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