Team:Warsaw/Calendar-Main/6 May 2008

From 2008.igem.org

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<h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br>
 
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Digestion was conducted with BamHI</h3>
 
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<center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center>
 
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Revision as of 20:37, 22 September 2008

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1. Preparation of electro- and chemocompetent bacteria E. coli TOP10 (lot of fun)
2. Isolation of genomic DNA (E.coli Rosetta)
3. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320)